LURIA BROTH (Miller´s LB Broth)

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LURIA BROTH (Miller´s LB Broth)

Dehydrated powder for the preparation of nutritionally rich liquid medium for the growth and maintenance of recombinant strains of Escherichia coli in molecular biology studies.
Quantity: 500g
Appearance: Beige powder. Autoclaved medium should be clear amber.
Storage: 2ºC – 25ºC. When not in use, keep container closed to avoid hydration.

Formulation (g/L)
Tryptone: 10,00
Yeast Extract: 5,00
NaCl: 10,00
Final pH (25ºC): 7,0 ± 0,2

Preparation:
Add 25g of the dehydrated medium to one liter of distilled water. Mix well and dissolve by heating with regular agitation. Boil for 1 minute in order to dissolve completely. Dispense in appropriate containers and sterilize by autoclaving at 121ºC for 15 to 20 minutes. Store at 2ºC to 8ºC.

Supplements
Luria Broth (Miller´s LB Broth) is a rich growth medium, which contains all the nutritional requirements for E.coli. Tryptone and Yeast Extract are the sources for carbon, nitrogen, vitamins, minerals, and amino acids essential for growth, whereas sodium chloride supplies essential electrolytes for transport and osmotic balance. For faster growth, medium can be supplemented with glucose (0.1%) or glycerol (0.4%). Many  supplements, including antibiotics, are heat-sensitive and cannot be autoclaved. These should be filter-sterilized and added to the medium after it has cooled down.

 

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Category: Microbiology Culture Media