2xYT Agar

|

2xYT Agar

Dehydrated powder for the preparation of nutritionally rich solid medium (plates) for the growth and maintenance of recombinant strains of Escherichia coli in molecular biology studies and for growth of filamentous phage.

Quantity: 500g
Appearance: Beige powder. Autoclaved medium should be amber, slightly opalescent.
Storage: 2ºC – 25ºC. When not in use, keep container closed to avoid hydration.

Formulation (g/L)
Tryptone: 16,00
Yeast Extract: 10,00
NaCl: 5,00
Bacteriological Agar: 15.00
Final pH (25ºC): 7,0 ± 0,2

Preparation:
Add 46g of the dehydrated medium to one liter of distilled water. Mix well and dissolve by heating with regular agitation. Boil for 1 minute in order to dissolve completely. Dispense in appropriate containers and sterilize by autoclaving at 121ºC for 15 to 20 minutes. Cool to 45-50ºC and dispense into plates. Plates should be stored at 8ºC to 15ºC.

Supplements
2xYT Agar (2xTY Agar) is a rich growth medium, optimized for growth and maintenance of ilamentous phage such as M13. It contains all the nutritional requirements for E.coli. Tryptone and Yeast Extract are the  sources for carbon, nitrogen, vitamins, minerals, and amino acids essential for growth, as well as growth factors that allow phages to reproduce without weakening the host cells. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Many supplements, including antibiotics, are heat-sensitive and cannot be autoclaved. These should be filter-sterilized and added to the medium after it has cooled down and prior to solidification.

Tags: , ,

Category: Microbiology Culture Media